A A Email Print Share

Christopher K. Surratt, Ph.D.

Professor
Mylan School of Pharmacy
Pharmaceutical Sciences

437 Mellon Hall
Phone: 412.396.5007

Education:

Ph.D., Chemistry, University of Virginia, 1988
B.A., Chemistry, University of Virginia, 1983
Bio

 

Graduate work in the Department of Chemistry at the University of Virginia, under the direction of Prof. Sidney M. Hecht, focused on control of tRNA aminoacylation and RNase P processing.  A secondary project studied cleavage of DNA by the antitumor agent bleomycin.  A postdoctoral fellowship at UC Berkeley followed, in the lab of Prof. Michael J. Chamberlin in the Department of Biochemistry.  This work focused on the mechanism of action of bacterial RNA polymerases.  Intrigued by molecular neuroscience, I accepted a Senior Staff Fellow position with the National Institute on Drug Abuse (NIDA) in 1991.  That year marked the start of a cloning frenzy of DNAs encoding brain receptors for drugs of abuse, antidepressants and other psychoactive agents.  NIDA's Laboratory of Molecular Neurobiology cloned some of the first opiate and psychostimulant receptors, and I created and characterized the first mutants of the heroin/morphine (mu opioid) receptor toward elucidation of the receptor's binding sites for abused opiates.  Later work at NIDA involved similar studies on the dopamine transporter (DAT), the brain's -cocaine receptor-.  I moved my studies to the Albert Einstein College of Medicine in 1998, as an assistant professor of neuroscience and psychiatry.  Primarily because of its teacher-scholar model, I moved to Duquesne University in 2001.  The DAT work became my research priority, as no FDA-approved anti-psychostimulant therapeutics were available then (and remain elusive even now).  Our DAT work has identified amino acid residues in this protein key for cocaine binding, and that allow discrimination of abused and non-abused psychostimulants.  The work has more recently extended to the serotonin and norepinephrine transporter proteins, with the goal of discovering and developing novel antidepressant and anti-anxiety medications.

Research

 

Structure-function studies on the plasma membrane monoamine (dopamine, norepinephrine and serotonin) transporter (MAT) proteins are the primary research focus. Computer molecular modeling-guided site-directed mutagenesis of transporter amino acid residues and molecular pharmacology of the obtained mutant transporters are employed in elucidating binding sites for abused psychostimulants (e.g., cocaine, amphetamine), antidepressants and other CNS drugs. The molecular models are also used in virtual screening of small molecule structural libraries in search of novel MAT ligands. Such ligands are then tested as candidate -lead compound- therapeutics for a variety of CNS disorders. Our molecular pharmacology lab works closely with computational chemists, medicinal chemists, biophysical chemists and behavioral neuropharmacologists at Duquesne University, University of Pittsburgh, NIH and several other research institutes.

Scholarship

Grants and Contracts (2006 - Present)
- 5R01DA026530 National Institute on Drug  Abuse (NIDA) NIH Grant
 Principal Investigator: C. K. Surratt, Ph.D.
 -Dopamine Transporter Structure and Function-
 $627,250 7/1/09 - 6/30/12
- R01DA027806 NIDA NIH/NSF Grant
 Principal Investigator: J. D. Madura, Ph.D.
-CRCNS: Computational and Experimental Study of Dopamine and Serotonin Transporters'
 $1,545,450 7/1/09 - 6/30/14
- R03DA027081 NIDA NIH Grant
Principal Investigator: D. J. Lapinsky, Ph.D.
-Non-Tropane Irreversible Dopamine Transporter Ligands'
$364,182 7/1/09 - 6/30/11
- 2R15DA016604-03A1, National Institute on Drug Abuse (NIH) Grant
 Principal Investigator: C. K. Surratt, Ph.D.
 -Monoamine Transporter Structure-Function Studies'
 $179,019 8/1/07 - 7/31/10
- Hunkele Dreaded Disease Award
 Principal Investigator: D. J. Lapinsky, Ph.D.
"Molecular Modeling-Guided Discovery and Design of Novel Irreversible Ligands for the Dopamine Transporter, the Brain's -Cocaine Receptor-"
 $10,000 in direct costs 7/1/08 - 6/30/09
- 1R15DA016604-01, National Institute on Drug Abuse (NIH) Grant
 Principal Investigator: C. K. Surratt, Ph.D.
 -Dopamine Transporter Structure-Function Studies'
 $116,660 approved, $90,150 disbursed 8/1/03 - 7/31/06
- 1R15DA016604-01S1, National Institute on Drug Abuse (NIH) Supplemental Grant
 Principal Investigator: C. K. Surratt, Ph.D.
 -Dopamine Transporter Structure-Function Studies'
 $77,914 8/1/03 - 7/31/06
- Samuel & Emma Winters Foundation grant
 Principal Investigator: C. K. Surratt, Ph.D.
 "Structure-Function Studies on a Cocaine and Amphetamine Receptor"
 $11,000 in direct costs 7/1/05 - 9/30/06
- Nobel J. Dick Faculty Development Fund grant
 Principal Investigator: C. K. Surratt, Ph.D.
 "Unraveling Molecular Mechanisms of Psychostimulant Drugs of Abuse"
 $10,000 in direct costs 7/1/05 - 6/30/06
Publications (2006 - Present)
Lapinsky, D.J., Yarravarapu, N., Nolan, T.L., Surratt, C.K., Lever, J.R., Tomlinson, M., Vaughan, R.A. and Deutsch, H.M. (2012). Evolution of a compact photoprobe for the dopamine transporter based on (-)-threo-methylphenidate (Ritalin, Concerta).  ACS Med. Chem. Lett., published online 3/12/12.
Nolan, T.L., Lapinsky, D.J., Talbot, J.N., Indarte, M., Liu, Y., Manepalli, S., Geffert, L.M., Amos, M.E., Taylor, P.N., Madura, J.D. and Surratt, C.K. (2011). Identification of a novel selective serotonin reuptake inhibitor by coupling monoamine transporter-based virtual screening and rational molecular hybridization. ACS Chem. Neurosci., 2, 544-52.
Manepalli, S., Geffert, L.M., Surratt, C.K. and Madura, J.D. (2011). Discovery of novel selective serotonin reuptake inhibitors through development of a protein-based pharmacophore. J. Chem. Inf. Model. 51, 2417-26.
Lapinsky, D.J., Aggarwal, S., Nolan, T.L., Surratt, C.K., Lever, J.R., Acharya, R., Vaughan, R.A., Pandhare, A. and Blanton, M.P.: (-)-2-(N-tert-butylamino)-3'-[125I]-iodo-4'-azidopropiophenone: a dopamine transporter and nicotinic acetylcholine receptor photoaffinity ligand based on bupropion (Wellbutrin, Zyban). Bioorg. Med. Chem. Lett., published online 11/24/11 (PMID: 22119468).
Surratt, C.K., Wildfong, P.L.D. and Kamal, K. (2010). Research funding expectations as a function of faculty teaching/administrative workload. Res. Soc. Admin. Pharmacy, published online 7/23/10 (DOI:10.1016/j.sapharm.2010.04.006).
Van Amburgh, J.A., Surratt, C.K., Green, J., Gallucci, R.M., Colbert, J., Zatopek, S.L. and Blouin, R.A. (2010). Succession planning in U.S. pharmacy schools. Am. J. Pharm. Educ. 74, Article 86 (online).
Indarte, M., Liu, Y., Madura, J.D. and Surratt, C.K. (2010). Receptor-based discovery of a plasmalemmal monoamine transporter inhibitor via high-throughput docking and pharmacophore modeling. ACS Chem. Neurosci. 1, 223-33.
Gedeon, P., Indarte, M., Surratt, C.K. and Madura, J.D. (2010). Molecular dynamics of leucine and dopamine transporter proteins in a model cell membrane lipid bilayer. Proteins 78, 797-811.
Lapinsky, D.J., Aggarwal, S., Huang, Y., Surratt, C.K., Lever, J.R., Foster, J.D. and Vaughan, R.A. (2009). A novel photoaffinity ligand for the dopamine transporter based on pyrovalerone. Bioorg. Med. Chem. 17, 3770-4.
Indarte, M., Madura, J.D. and Surratt, C.K. (2008). Dopamine transporter comparative molecular modeling and binding site prediction using the LeuTAa leucine transporter as a template. Proteins. 70, 1033-46. (Published online 9/10/07.)
Surratt, C.K. and Desselle, S.P. (2007). Pharmacy students' perception of a teaching evaluation process. Am. J. Pharm. Educ., 71, Article 06 (online).
Ukairo, O.T., Ramanujapuram, S. and Surratt, C.K. (2007). Fluctuation of the dopamine uptake inhibition potency of cocaine, but not amphetamine, in mammalian cells expressing the dopamine transporter. Brain Res., 1131, 68-76.
Surratt, C.K., Witt-Enderby, P.A., Johnson, D.A., Anderson, C.A., Bricker, J.D., Davis, V.L., Firestine, S.M. and Meng, W.S. (2006). Development of a neuroscience oriented "methods" course for graduate students of pharmacology and toxicology. Cell Biol. Educ. 5, 188-96.
Surratt, C.K. (2006). Creation of a graduate oral/written communication skills course. Am. J. Pharm. Educ., 70(1):Article 05, 1-8
Presentations (2006 - Present)
Podia:
"Using TurningPoint personal response systems as a teaching tool in large lecture classes", PCHE e-learning seminar series, Duquesne University, March 2006.
-The neurochemical basis of drug addiction-, Chemical Dependency Awareness Program, Duquesne University School of Pharmacy, January 2008.
-The neuroscience behind drugs of abuse-, Duquesne University School of Education, March 2008.
-Molecular modeling-guided elucidation of dopamine transporter ligand binding sites: development of an in silico -cocaine antagonist- screening tool-, College on Problems of Drug Dependence annual meeting, San Juan, PR, June 2008.
 -Getting funded-, West Virginia University School of Pharmacy, Morgantown, WV, May 2009.
-Monoamine uptake inhibitor interactions in the primary and 'staging area- substrate pockets of the plasma membrane monoamine transporters', College on Problems of Drug Dependence annual meeting, Reno, NV, June 2009.
'succession planning in U.S. pharmacy schools', American Association of Colleges of Pharmacy annual meeting, Boston, MA, July 2009.
-The neurochemical basis of drug addiction-, Chemical Dependency Awareness Program, Duquesne University School of Nursing, October 2009.
-Discovery of potential CNS therapeutic lead compounds via high-throughput small molecule virtual docking to monoamine transporter models', NeuroTalk 2010 conference, Singapore, June 2010.
-Drug discovery approaches using computer molecular models of monoamine transporter proteins', School of Biological Sciences, Victoria University of Wellington (New Zealand), June 2010.
-Drug discovery approaches using computer molecular models of monoamine transporter proteins', Department of Pharmacology, University of Sydney Medical School, Sydney, Australia, July 2010.
-Discovery of a monoamine transporter inhibitor via high-throughput molecular model docking and pharmacophore modeling-, 16th World Congress of Basic and Clinical Pharmacology (WorldPharma2010), Copenhagen, Denmark, July 2010.
"Annual performance reviews: steps toward promotion and tenure", 2011 AACP Interim Meeting, Savannah, GA, February 2011. 
Symposia organized and chaired:
-Virtual modeling: A new frontier for investigating drug-receptor interactions', College on Problems of Drug Dependence, Reno, NV, June 2009.
-Track 4-4: Ion channels, transporters and neurotransmitter receptors', NeuroTalk 2010 conference, Singapore, June 2010.
Posters:
Indarte, M., Heyman, J., Madura, J.D., Liu, Y., Adams, W.R. and Surratt, C.K. (2006).  Melatonin receptor binding pocket insights based on de novo molecular modeling.  Experimental Biology conference, San Francisco (online).
Ramanujapuram, S., Ukairo, O.T. and Surratt, C.K. (2006).  Fluctuation of the dopamine uptake inhibition potency of cocaine, but not amphetamine, in CHO cells stably transfected with the dopamine transporter.  Experimental Biology conference, San Francisco (online).
Indarte, M., Madura, J.D. and Surratt, C.K. (2006).  Comparative molecular modeling of the dopamine transporter using the leucine transporter LeuTAa as a template.  Society for Neuroscience Abstracts (online).
Desselle, S.P., Conklin, M.H., Surratt, C.K., Astle, J., Skomo, M.L., Berdine, H.J., O-Neil, C.K. and Mihalyo, M.G. (2006).  Use of a service-learning experience to bridge knowledge between professional courses. American Association of Colleges of Pharmacy Annual Meeting, San Diego, CA.
Madura, J.D., Indarte, M. and Surratt, C.K. (2007).  Comparative modeling and ligand binding of the dopamine transporter. American Chemical Society meeting, Chicago (online).
Indarte, M., Madura, J.D. and Surratt, C.K. (2007).  Comparative models of dopamine and serotonin transporter proteins.  Chemical Computing Group conference, Montreal (online).
Huang, Y., Liu, Y., Madura, J.D. and Surratt, C.K. (2008).  Electrostatic binding free energy calculations for drug-neurotransmitter transporter protein interactions.  Chemical Computing Group conference, Montreal (online).
Manepalli, S., Madura, J.D., Indarte, M. and Surratt, C.K. (2008).  Using a computational serotonin transporter model to identify binding site residues.  Chemical Computing Group conference, Montreal (online).
Surratt, C.K., Indarte, M., Madura, J.D., Liu, Y. and Huang, Y. (2008).  Molecular modeling-guided elucidation of dopamine transporter ligand binding sites: development of an in silico -cocaine antagonist- screening tool.  College on Problems of Drug Dependence, San Juan, PR.
Manepalli, S., Madura, J.D., Lapinsky, D.J. and Surratt, C.K. (2009). Elucidation of binding profile similarities across structurally diverse ligands using a 3D dopamine transporter model.  American Chemical Society meeting, Washington, DC (online).
Huang, Y., Liu, J., Aggarwal, S., Indarte, M., Manepalli, S., Madura, J.D., Surratt, C.K. and Lapinsky, D.J. (2009).  Characterization of novel pyrovalerone analogs as potential dopamine transporter irreversible ligands.  FASEB Experimental Biology conference, New Orleans, LA (online).
Surratt, C.K., Indarte, M., Manepalli, S., Madura, J.D., Liu, Y., Huang, Y., Lapinsky, D.J. and Aggarwal, S. (2009). Virtual modeling: A new frontier for investigating drug-receptor interactions.  College on Problems of Drug Dependence, Reno, NV.
Van Amburgh, J.A., Zatopek, S.L., Colbert, J., Gallucci, R.M., Green, J. and Surratt, C.K. (2009). Succession planning in U.S. pharmacy schools. American Association of Colleges of Pharmacy, Boston, MA.
Aggarwal, S., Lapinsky, D.J., Huang, Y., Surratt, C.K., Lever, J.R., Foster, J.D., Vaughan, R.A., Manepalli, S. and Madura, J.D. (2009).  Development of novel photoaffinity ligands for the dopamine transporter based on pyrovalerone. American Chemical Society meeting, Washington, DC (online).
Madura, J.D., Lapinsky, D.J. and Surratt, C.K. (2010). Structure, function and dynamics of monoamine transporter proteins.  Collaborative Research in Computational Neuroscience conference, Baltimore (online).
Nolan, T., Gofman, A., Surratt, C.K. and Madura, J.D. (2010). Prediction of norepinephrine transporter substrate and inhibitor binding pockets via homology modeling and ligand docking score validation.  Chemical Computing Group conference, Montreal (online).
Geffert, L., Manepalli, S., Lapinsky, D., Madura, J.D. and Surratt, C.K. (2010).  Pharmacologic validation of MOE docking scoring of novel serotonin transporter inhibitors.  Chemical Computing Group conference, Montreal (online).
Surratt, C.K. (2010). Discovery of potential CNS therapeutic lead compounds via high-throughput small molecule virtual docking to monoamine transporter models. NeuroTalk 2010 conference, Singapore (online).
Surratt, C.K., Kamal, K. and Wildfong, P.L.D. (2010).  Research funding expectations as a function of faculty teaching/administrative workload. American Association of Colleges of Pharmacy, Seattle, WA.
Van Amburgh, J.A., Surratt, C.K., Green, J., Gallucci, R.M., Colbert, J., Zatopek, S.L. and Blouin, R.A. (2010). Succession planning in U.S. pharmacy schools. American Association of Colleges of Pharmacy, Seattle, WA.
Surratt, C.K., Indarte, M., Liu, Y. and Madura, J.D. (2010). Discovery of a monoamine transporter inhibitor via high throughput molecular model docking and pharmacophore modeling. IUPHAR 16th World Congress of Basic and Clinical Pharmacology, Copenhagen, DK (online).
Yarravarapu, N., Lapinsky, D.L., Velagaleti, R., Liu, Y., Nolan, T., Surratt, C.K., Madura, J.D., Lever, J.R., Foster, J.D. and Vaughan, R.A. (2010).  Development of novel photoaffinity probes for the dopamine transporter based on methylphenidate (Ritalin, Concerta).  Mid-Atlantic Graduate Student Symposium on Medicinal Chemistry, Purdue University, West Lafayette, IN.
Lapinsky, D., Surratt, C., Madura, J., Lever, J. and Vaughan, R. (2010).  Development of novel photoaffinity ligands for the dopamine transporter based on methylphenidate (Ritalin).  20th Neuropharmacology Conference (satellite of Soc. Neuroscience), San Diego, CA, Abstract P1.16.
Geffert, L.M., Huang, Y., Nolan, T.L., Indarte, M., Manepalli, S., Madura, J.D. and Surratt, C.K. (2011). Characterization of the extracellular vestibule of the human serotonin transporter.  College on Problems of Drug Dependence, Miami, FL, Abstract 237.
Nolan, T.L., Lapinsky, D.J., Indarte, M., Liu, Y., Madura, J.D. and Surratt, C.K. (2011). Discovery of a novel SSRI scaffold from a lead compound identified by in silico screening.  College on Problems of Drug Dependence, Miami, FL, Abstract 524.
Surratt, C.K., Indarte, M., Liu, Y., Madura, J.D. and Talbot, J.N. (2011). Preclinical antipsychostimulant and antidepressant properties of a monoamine transporter ligand identified via in silico screening.  College on Problems of Drug Dependence, Miami, FL, Abstract 699.
Surratt, C.K., Nolan, T.L., Lapinsky, D.J., Talbot, J.N., Indarte, M., Manepalli, S., Geffert, L.M. and Madura, J.D. (2011). Virtual screening for antidepressant lead compounds using monoamine transporter computational models. Collaborative Research in Computational Neuroscience meeting, Princeton Neuroscience Institute, Princeton University, Princeton, NJ, Oct. 9, 2011.
Nolan, T.L., Lapinsky, D.J., Talbot, J.N., Indarte, M., Liu, Y., Manepalli, S., Geffert, L.M., Amos, M.E., Taylor, P.N., Madura, J.D. and Surratt, C.K. (2011). Development of a novel SSRI via structure-based virtual screening and rational molecular hybridization. Anxiety and Depression: 21st Neuropharmacology Conference, Washington, DC, Nov. 10, 2011.
Talbot, J.N., Tanda, G., Katz, J.L., Indarte, M., Mereu, M., Madura, J.D. and Surratt, C.K. (2011). Preclinical antidepressant and antipsychostimulant properties of a novel triple reuptake inhibitor lead compound identified via virtual screening. Anxiety and Depression: 21st Neuropharmacology Conference, Washington, DC, Nov. 10, 2011.

Courses

PHBM 352: Foundations of Pharmacology and Medicinal Chemistry (Coursemaster)
PHSL 477W: Service Learning Experience in Pharmacy
GPSC 513: Principles of Drug Action, Design and Delivery
GPSC 572: Methods of Evaluation of Drug Action and Toxicity

Honors/Awards

2011 NIH Study Section MCDN (Molecular, Cellular and Developmental Neuroscience) Special Emphasis Panel reviewer (R01 grants)
2010 NIH Study Section F02A (Behavioral Neuroscience Fellowship) ad hoc reviewer 
2010 and 2011 NIH Study Section GXM-A (Molecular Pharmacology - Cutting Edge Basic Research Award R21 grant) reviewer
2010 NIH Study Section ZRG1 MDCN-G (Molecular, Cellular and Developmental Neuroscience - Program Project Grants) primary P01 reviewer; also reviewed individual project grants within the P01
2009 - 2010 Academic Leadership Program Fellow, Duquesne University (inaugural year)
2008 - 2009 Apple Polishing Award for excellence in teaching and service, Duquesne University Greek Life
2008 American Association of Colleges of Pharmacy Academic Leadership Fellow (30 awards nationwide)
2006 Presidential Award for Excellence in Teaching, Duquesne University
2005 - 2006 Creative Teaching Award, Duquesne University
2004 - 2005 Excellence in Teaching Award, School of Pharmacy, Duquesne University
2004 - 2005 Excellence in Teaching Award, Division of Pharmaceutical Sciences, Duquesne University
2004 - 2005 Assessment of Student Learning Award, Division of Pharmaceutical Sciences, Duquesne University
2004 - 2005 Excellence in Service Award, Division of Pharmaceutical Sciences, Duquesne University
2003 - 2004 Excellence in Research Award, Division of Pharmaceutical Sciences, Duquesne University
2003 American Association of Colleges of Pharmacy Teacher of the Year (124 professors awarded nationwide)
Nominee, 2003 Teacher of the Year, Omicron Delta Kappa National Leadership Society
2002 - 2003 Excellence in Teaching Award, Division of Pharmaceutical Sciences, Duquesne University
1997 NIH Fellows Award for Research Excellence
1994 Abstract Award, Society for Neuroscience, Baltimore Chapter