Wilson Meng, Ph.D.Associate Professor
Mylan School of Pharmacy
Education:Ph.D., Pharmaceutical Sciences, University of Southern California, 1998
B.S., Pharmacy, University of Maryland, 1991
Wilson Meng received his Ph.D. in Pharmaceutical Sciences from the University of Southern California. After completing a postdoctoral fellowship at the UCLA School of Medicine, he joined Duquesne University in 2001. He is currently Associate Professor of Pharmaceutical Sciences. He is also Visiting Scholar at the Molecular Biosensor and Imaging Center at Carnegie Mellon University. He has served as grant reviewers for the National Institutes of Health, The Wellcome Trust (UK), and the National Science Foundation. He is a member of the editorial board for the Journal of Pharmaceutical Innovation (Springer), and regularly reviews manuscripts for Biomaterials and International Journal of Pharmaceutics. From 2003 to 2007 he served on the AAPS Student/Post Doc Outreach and Development (SPOD) Committee. He graduated from the University of Maryland School of Pharmacy and practiced community pharmacy in Maryland and California.
Research foci: Design and characterization of bioinspired DNA/protein delivery systems to modulate immune functions
My group has developed particles that combine the polymer PLGA and the polycation O10H6 as nucleic acid delivery systems (Chamarthy et al, 2003, 2004; Kovacs et al, 2005; Zheng et al, 2006; Jia et al, 2006, 2008; Kovacs et al 2009). These studies support the notion that PLGA can be used in the context of generating tolerogenic dendritic cells. We are testing PLGA-O10H6 particles to deliver the interleukin-10 gene in a mouse transplant model (funded by NIH). In collaboration with Nick Giannoukakis (Pitt) we have formulated PLGA particles loaded with immune active factors to suppress asthmatic inflammation (US patent pending) and to delay the onset of type-I diabetes in mice (funded by JDRF). Working with Lisa Weiland (Pitt) and Eric Freeman (VTech), we have constructed a mathematical model predictive of amine-induced endosomal burst (Freeman et al, 2010, 2012). Together with Mat Saunders and Alan Waggoner at Carnegie Mellon we are developing injectable biosensors for drug delivery applications. We maintain an interest in T cell epitope prediction (Joseph et al, 2007). More recently, my group has characterized injectable peptidic membranes (functionalized EAK16-II) to extend the accumulation of antibodies in tumors (Zheng et al, 2011; funded by PA-CURE). In many of these projects we work closely with our Duquesne chemistry colleague Ellen Gawalt.
JDRF 17-2012-348 (Co-PI: Meng; PI: Giannoukakis of Children's Hospital of Pittsburgh); 9/1/2012-8/30/2013; Subcontract award $49,567
Project title: Type 1 Diabetes-Suppressive Microspheres
NIH R15 AI081218 (PI: Meng); 12/1/2010-11/30/2013; total award: $357,100
Project title: Genetic Modification of Antigen-Presenting Cells in Allograft
CURE-Pennsylvania Department of Health (PI: Meng); 1/1/2012-12/31/2013; total award: $53,732
Project Title: A Biomaterial Approach to Inhibit Melanoma Growth and Metastasis in Mice
CURE-Pennsylvania Department of Health (PI: Meng); 1/1/2007-12/31/2010; total award: $35,660
Project Title: Modulation of Antitumor Immunity with Anti-Foxp3 siRNA Nanoparticles
NIH R15 CA97990 (PI: Meng); 03/10/2004-02/28/2008; total award: $192,005
Project title: Rational Design of Peptide-Based Tumor Vaccines
Saunders MJ, Liu W, Szent-Gyorgyi C, Wen Y, Drennen Z, Waggoner AS, and Meng WS. 2013. "Engineering Fluorogen Activating Proteins into Self-Assembling Materials." Bioconjugate Chemistry, in press
Wen Y, Kolonich HR, Kruszewski KM, Giannoukakis N, Gawalt ES, Meng WS. 2013. "Retaining Antibodies in Tumors with a Self-Assembling Injectable System." Molecular Pharmaceutics. 10:1035-44
Freeman EC, Weiland LM, and Meng WS. 2013. "Modeling the Proton Sponge Hypothesis: Examining Proton Sponge Effectiveness for Enhancing Intracellular Gene Delivery Through Multiscale Modeling." J Biomaterials Science, Polymer Edition, 24:398-416
Balducci A, Wen Y, Zhang Y, Helfer BM, Hitchens TK, Meng WS, Wesa AK, and Janjic JM. 2013. "A Novel Probe for the Non-Invasive Detection of Tumor-Associated Inflammation." Oncoimmunology. 2:e23034
Zheng, Y., Wen, Y., George, A.M., Steinbach, A., Freeman, E., Philips, B., Giannoukakis, N., Weiland, L.M., Gawalt, E.S., and Meng, W.S.* 2011. 'A Peptide-Based Material Platform for Displaying Antibodies to Engage T Cells' Biomaterials, 32, 249-257
Freeman, E.C, Weiland, L.M. & Meng, W.S. 2010 -Application of Proteins in Burst Delivery Systems.' Smart Materials and Structures. 19, 94015-94024
Kovacs, J.R., Tidball, J., Ross, A., Jia, L., Zheng, Y., Gawalt, E.S. and Meng, W.S. 2009 -Characterization of Nickel-Decorated Particles Anchored with a His-tagged Polycation.' Journal of Biomaterials Science, Polymer Edition, 20(9): 1307-1320.
Jia, L, Kovacs, J.R., Zheng, Y., Shen, H., Gawalt, E.S. and Meng, W.S* 2008. 'Expansion of Foxp3-expressing Regulatory T Cells in vitro from Dendritic Cells Engineered with Polymeric Particles Carrying Plasmid Encoding Interleukin-10.' Biomaterials, 29(9):1250-61.
Joseph, M.A., Mitchell, L.M., Evanseck, J.D., Kovacs, J.R., Jia, L., Shen, H., and Meng, W.S.* 2007 'secondary Anchor Substitutions in a HLA-A*0201-Restricted T Cell Epitope Derived from Her-2/neu- Molecular Immunology, 44(4):322-31.
Zheng, Y., Kovacs, J.R., Gawalt, E.S., Shen, H., and Meng, W.S.* 2006. 'Characterization of Particles Fabricated with Poly (D, L-lactic-co-glycolic acid) and an Ornithine-Histidine Peptide as Carriers of Oligodeoxynucleotide for Delivery into Primary Dendritic Cells' Journal of Biomaterials Science, Polymer Edition, 17(12): 1389-1403.
Jia, L., Kovacs, J.R., Zheng, Y., Gawalt, E.S., Shen, H., and Meng, W.S.* 2006. 'Attenuated Alloreactivity of Dendritic Cells Engineered with Surface-Modified Microspheres Carrying a Plasmid Encoding Interleukin-10.' Biomaterials, 27(9):2076-2082.
Meng, W.S.*and Butterfield, L.H. 2005. 'Activation of Antigen-Presenting Cells by DNA Delivery Vectors.' (Review) Expert Opinion on Biological Therapy, 5(8), 1019-1028.
Kovacs, J.R., Zheng, Y., Shen, H., and Meng, W.S*. 2005. 'Polymeric Microspheres as Stabilizing Anchors for Oligonucleotide Delivery to Dendritic Cells.' Biomaterials, 26(33), 6754-61.
Liao, Y-P., Wang, C-C., Butterfield, L.H., Economou, J.S., Ribas, A., Meng, W.S., Iwamoto, K.S., and McBride, W.H. 2004. 'Ionizing Radiation Affects Human MART-1 Melanoma Antigen Processing and Presentation by Dendritic Cells.' Journal of Immunology, 173, 2462-2469.
Chamarthy, S.P., Jia, L., Kovacs, J.R., Anderson, K.R., Shen, H., Firestine, S.M., and Meng, W.S*. 2004. 'Gene Delivery to Dendritic Cells Facilitated by a Tumor Necrosis Factor Alpha-Competing Peptide.' Molecular Immunology, 41 (8), 741-749.
Butterfield, L.H., Ribas, A. Meng, W.S., Dissette, V.B., Amarnani, S. Vu, H.T., Seja, E., Todd, K., Glaspy, J.A., McBride W.H. and Economou, J.S. 2003. 'T Cell Responses To HLA-A*0201 Immunodominant Peptides Derived From Alpha Fetoprotein in Patients with Hepatocellular Cancer.' Clinical Cancer Research, 9(16), 5902-5908.
Chamarthy, S.P., Kovacs, J.R., Gattens, D., McClelland, E., and Meng, W.S*. 2003. 'A Cationic Peptide Consists of Ornithine and Histidine Repeats Augments Gene Transfer in Dendritic Cells.' Molecular Immunology, 40 (8), 479-486.
Meng, W.S.* and Butterfield, L.H. 2002. 'Rational Design of Peptide-Based Tumor Vaccines.' (Review) Pharmaceutical Research. 19 (7): 926-932.
Meng, W.S., Butterfield, L.H., Ribas, A., Dissette, V., Heller, J.B., Miranda, G.A., Glaspy, J.A., McBride, W.H. and Economou, J.S. 2001. 'Alpha Fetoprotein-Specific Tumor Immunity Induced by Plasmid Prime-Adenovirus Boost Genetic Vaccination.' Cancer Research, 61 (24): 8782.
Ribas, A., Butterfield, L.H., Amarnani, S., Dissette, V.B., Kim, D., Meng, W.S., Miranda, G.A., Wang, H-J., McBride, W.H., Glaspy, J.A., and Economou, J.S. 2001. 'CD40L Crosslinking Bypasses the Absolute Requirement for CD4 Cells During Immunization with Melanoma Antigen Gene-Modified Dendritic Cells.' Cancer Research. 61 (24):8787.
Meng, W.S., Butterfield, L.H., Ribas, A., Heller, J.B., Dissette, V., Glaspy, J.A., McBride, W.H. and Economou, J.S. 2001. 'Fine specificity analysis of an HLA-A*0201-restricted immunodominant peptide derived from human alpha-fetoprotein.' Molecular Immunology, 2001. vol. 37, 943-950.
Butterfield, L.H., Meng, W.S., Koh, A., Vollmer, C.M., Ribas, A., Dissette, V., Lee, E., Faull, K., Glaspy, J.A., McBride, W.H. and Economou, J.S. 2001. 'Generation of human T-cell responses to strong binding HLA-A2.1-restricted peptide epitopes derived from alpha-fetoprotein.' Journal of Immunology, vol. 166, 5300-5309.
Meng, W.S., von Grafenstein, H., and Haworth, I.S. 2000. 'Water Dynamics at the interface of four different MHC-peptide complexes.' International Immunology, vol. 12, no. 7, 949-957.
Meng, W.S., Bui, H-H., and Haworth, I.S. 2000. 'Exploiting the Peptide-MHC Water Interface in the Computer-Aided Design of Non-Natural Peptides that Bind to the Class I MHC Molecule HLA-A2.' Molecular Simulation, vol. 24, 215-228.
Ribas, A., Butterfield, L.H., Hu, B, Dissette, V.B., Meng, W.S., Koh, A., Andrews, K.J., Lee, M., Amar, S.N., Glaspy, J.A., McBride, W.H., and Economou, J.S. 2000. 'Immune deviation and Fas-mediated deletion limit antitumor activity after multiple dendritic cell vaccinations in mice.' Cancer Research, vol. 60, n.8: 2218-2224.
Gurlo, T., Meng, W.S., Bui, H-H., Haworth, I.S. and von Grafenstein, H. 1999. 'Experimental Evidence for the Presence of a Water Network at the Peptide-MHC Interface.' Immunology Letters, vol. 70:139-41.
Butterfield, L.H., Koh, A., Meng, W., Vollmer, C.M., Ribas, A., Dissette, V., Lee, E., Glaspy, J.A., McBride, W.H. and Economou, J.S. 1999. 'Generation of human T-cell responses to an HLA-A2.1-restricted peptide epitope derived from alpha-Fetoprotein.' Cancer Research, vol.59, n.13: 3134-3142.
Meng, W.S., Bhavaraju, A.V., Haworth, I.S. and von Grafenstein, H. 1998. 'Modeling of the Non-Obese Diabetic Mouse Class II MHC Molecule I-Ag7.' Protein and Peptide Letters, vol. 5, No. 2, 75-82.
Meng W.S., von Grafenstein, H. and Haworth, I.S. 1997. 'A Model of Water Structure Inside the HLA-A2 Peptide Binding Groove.' International Immunology, vol 9, No. 9, 1339-1346.
Meng W.S., Gallaher, T.K., von Grafenstein, H. and Haworth, I.S. 1996. 'sequence Dependent Conformational Motion of a Peptide Bound to a Class I MHC Molecule.' Protein and Peptide Letters, vol. 3, No. 1, 51-58.
Human Physiology and Pathology II: Hematology and Immunology (Coursemaster)
Biomedical Science and Therapeutics: Principles of Hematology, Oncology and Transplantation
Graduate Pharmaceutical Sciences:
Advanced Pharmaceutics: Pharmaceutical Biotechnology (Coursemaster)
Advanced Pharmacology (Coursemaster)
Drug Mechanisms and Actions
Methods in Pharmacology and Toxicology