Bayer School of Natural and Environmental Sciences

Research Interests

Phospholipid metabolism is intimately coupled to the physiological status of the cell. As cells and organelles change size and shape with events such as cell division, secretion, and membrane biogenesis, phospholipids are continually broken down and resynthesized in a controlled manner. The regulation of phospholipid biosynthesis and degradation must take into account not only the concentrations and locations of individual phospholipid species, but also the production of lipid-derived second messenger molecules, the transport of molecules across membranes, and the environmental conditions under which the cells are growing. Employing the powerful molecular genetic tools provided by the model eukaryote Saccharomyces cerevisiae, my research focuses on the mechanisms by which the cell coordinates phospholipid metabolism with the complex cellular events for which it is crucial. This work is supported by a grant from the National Institutes of Health.

Representative Publications

1. Patton-Vogt, J. (2007) Transport and metabolism of glycerophosphodiesters produced through phospholipid deacylation. Biochim. Biophys. Acta., 1771:337-342.

2. Boumann HA, J. Gubbens, M.C. Koorengevel, C.S. Oh, C.E. Martin, A.J. Heck, J. Patton-Vogt, S.A. Henry, B. de Kruijff, A.I. de Kroon (2006) Depletion of phosphatidylcholine in yeast induces shortening and increased saturation of the lipid acyl chains: evidence for regulation of intrinsic membrane curvature in a eukaryote. Mol. Biol. Cell. 17:1006-17.

3. Almaguer, C., E. Fisher, J. Patton-Vogt (2006) Posttranscriptional regulation of Git1p, the glycerophosphoinositol/glycerophosphocholine transporter of Saccharomyces cerevisiae. Curr Genet., 50(6):367-75.

4. Mariggio, S., C. Iurisci, J. Sebastia, J. Patton-Vogt and D. Corda (2006). "Molecular characterization of a glycerophosphoinositol transporter in mammalian cells." FEBS Lett 580(30): 6789-96.

5. Fisher, E., C. Almaguer, R. Holic, P. Griac, J. Patton-Vogt (2005) Glycerophosphocholine-dependent growth requires Gde1p (YPL110c) and Git1p in Saccharomyces cerevisiae. J. Biol. Chem. 280:36110-7.

6. Almaguer, C. Cheng, W. Nolder, C., and Patton-Vogt, J. Glycerophosphoinositol, a novel phosphate source whose transport is regulated by multiple factors in Saccharomyces cerevisiae. (2004) J. Biol. Chem. 279, 31937-31942.

7. Almaguer, C., Mantella, D., Perez, E., and Patton-Vogt, J. Inositol and phosphate regulate GIT1 transcription and glycerophosphoinositol incorporation in Saccharomyces cerevisiae. (2003) Eukaryotic Cell . 2, 729-736.

8. Dowd, S.R., Bier, M.E., and Patton-Vogt, J.L. Turnover of phosphatidylcholine in Saccharomyces cerevisiae (2001) J. Biol. Chem 276, 3756-3763.

9. Shirra, K.S., Patton-Vogt, J.L., Ulrich, A., Liuta-Tehlivets, O., Kohlwein, S.D., Henry, S.A., and Arndt, K.M. Inhibition of acetyl-CoA carboxylase activity restores expression of the INO1 gene in a snf1 mutant strain of Saccharomyces cerevisiae. (2001) Mol. Cell. Biol. 21, 5710-5722.

10. Srinivas, A., Patton-Vogt, J.L., Bruno, V., and Henry, S.A. A role for the major phospholipase D (Pld1p) in the secretory pathway in yeast. (1998) J. Biol. Chem 273, 16635-16638.

11. Patton-Vogt, J.L. and Henry, S.A. (1998) GIT1, a gene encoding a novel transporter for glycerophosphoinositol in Saccharomyces cerevisiae. Genetics 149, 1707-1715.

12. Henry, S.A. and Patton-Vogt, J.L. (1998) Genetic regulation of phospholipid metabolism in yeast. Progress in Nucleic Acid Research and Molecular Biology 61, 133-179. (Invited review)

13. Patton-Vogt, J.L., Griac, P., Srinivas, A., Bruno, V., Dowd, S., Swede, M. and Henry, S.A. (1997) Role of the yeast phosphatidylcholine/phosphatidylinositol transfer protein (Sec14p) in phosphatidylcholine turnover and INO1 regulation. J. Biol. Chem. 272, 20873-20883.

14. Patton, J. L., Pessoa-Brandao, L., and Henry, S. A. (1995) Production and utilization of an extracellular phosphatidylinositol catabolite, glycerophosphoinositol, by Saccharomyces cerevisiae. J. Bacteriol. 177, 3379-3385.