Contact Information

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Biography

Dr. Patton-Vogt joined Duquesne University in 2001. She has a BS from the University of Wyoming and a PhD from the University of Kentucky.  Prior to working at Duquesne she was a Post-Doctoral Research Assistant then a Research Scientist, both at Carnegie Mellon University in Pittsburgh. Dr. Patton-Vogt's current research interests focus on the molecular genetics of phospholipid metabolism in Saccharomyces cerevisiae and Candida albicans

Education

  • PhD, Biochemistry, University of Kentucky, 1991
  • BS, Biochemistry, University of Wyoming, 1985

Research Interests

1. Primary Research Focus: Phospholipid Metabolism in Saccharomyces cerevisiae

The main research focus in my laboratory involves events that occur within or near cellular membranes. I am interested in membrane lipid synthesis and degradation, and the fate of the resulting metabolites. These processes are complex, because as cells and organelles change size and shape with cell division, secretion, and membrane biogenesis, the various lipid classes must be broken down and resynthesized in a controlled manner. The regulation of this metabolism must take into account not only the concentrations and locations of individual lipids, but also the production of lipid-derived second messenger molecules, and the environmental conditions under which the cells are growing. Defects in lipid homeostasis can contribute to the development of several diseases, including obesity, diabetes, and cancer.

The production, transport, metabolism and function of a class of phospholipid metabolites called glycerophosphodiesters is an area of focus in my laboratory. We use the simple and genetically tractable eukaryotic organism, S. cerevisiae, for these studies. Glycerophosphodiesters, such as glycerophosphocholine and glycerophosphoinositol, are produced through phospholipase B-mediated cleavage of their precursor phospholipids. Once produced, the glycerophosphodiesters are transported across the cell membrane via specific transporters. My laboratory has characterized a number of genes involved in this metabolism in S. cerevisiae, including a permease (Git1), a glycerophosphodiesterase (Gde1), and an acyltransferase (Gpc1). The fundamental importance of this metabolism is illustrated by the fact that its basic components have been described in organisms and cell types spanning the biological spectrum. Although this metabolism is universal, many of its molecular details remain to be uncovered. A focus of the laboratory is to use biochemical and molecular genetic techniques to dissect the regulation and function of lipid turnover, metabolite recycling and membrane remodeling.

2. Secondary Research Focus: Phospholipid Metabolism in Candida albicans

Candida albicans is a pathogenic yeast of significant medical importance. The genome of C. albicans contains homologs of the genes involved in our metabolism of interest (see paragraph above). We are analyzing those genes and related processes for their roles in cellular physiology and virulence.

We acknowledge funding from NIH, PA Department of Health, and USDA for the above studies. Mass spectrometry instrumentation obtained through an NSF grant is utilized for these analyses.

School

  • King WR, Acosta-Zaldívar M, Qi W, Cherico N, Cooke L, Köhler JR, Patton-Vogt J. Glycerophosphocholine provision rescues Candida albicans growth and signaling phenotypes associated with phosphate limitation. mSphere. 2023 Oct 16:e0023123. doi: 10.1128/msphere.00231-23. PMID: 37843297
  • King WR, Singer J, Warman M, Wilson D, Hube B, Lager I, Patton-Vogt J. The glycerophosphocholine acyltransferase Gpc1 contributes to phosphatidylcholine biosynthesis, long-term viability, and embedded hyphal growth in Candida albicans. J Biol Chem. 2023 Dec 8;:105543. doi: 10.1016/j.jbc.2023.105543. [Epub ahead of print] PubMed PMID: 38072057
  • Hrach VL, King WR, Nelson LD, Conklin S, Pollock JA, Patton-Vogt, J (2023) The acyltransferase Gpc1 is both a target and an effector of the unfloded protein response in Saccharomyces cerevisiae. Journal of Biological Chemistry 299(7) DOI: https://doi.org/10.1016/j.jbc.2023.104884
  • Robinson BP, Hawbaker  S, Chiang A, Jordahl EM, Anaokar S, Nikiforov A, Bowman III RW, Ziegler P, McAtee CK, Patton-Vogt J, and O’Donnell  AF (2022) Alpha-arrestins Aly1/Art6 and Aly2/Art3 regulate trafficking of the glycerophosphoinositol transporter Git1 and impact phospholipid homeostasis. Biology of the Cell, 114(1):3-13.  DOI: 10.1111/boc.202100007
  • Wang J, Pan W, Nikiforov A, King W, Hong W, Li W, Han Y, Patton-Vogt J, Shen J, Cheng L (2021) Identification of two glycerophosphodiester phosphodiesterase genes in maize leaf phosphorus remobilization. The Crop Journal. 9 (1):95-108.
  • Patton-Vogt J, de Kroon AIPM (2020) Phospholipid turnover and acyl chain remodeling in the yeast ER. Biochim Biophys Acta Mol Cell Biol Lipids. 1865(1):S1388-1981(19)30076-9.
  • Anaokar S, Kodali R, Jonik B, Renne MF, Brouwers JFHM, Lager I, de Kroon AIPM, Patton-Vogt J (2019) The glycerophosphocholine acyltransferase Gpc1 is part of a phosphatidylcholine (PC)-remodeling pathwaythat alters PC species in yeast. J Biol Chem. 294:1189-1201.
  • Tams RN, Cassilly CD, Anaokar S, Brewer WT, Dinsmore JT, Chen YL, Patton-Vogt J, Reynolds TB (2019) Overproduction of Phospholipids by the Kennedy Pathway Leads to Hypervirulence in Candida albicans. Front Microbiol. 10:86.
  • Glab B, Beganovic M, Anaokar S, Hao M-S, Rasmusson AG, Patton-Vogt J, Banas´ A, Stymne S, and Lager L (2016) Cloning of Glycerophosphocholine Acyltransferase (GPCAT) from Fungi and Plants. J. Biol. Chem. 291:25066-25076.
  • Ding J, Holzwarth G, Penner MH, Patton-Vogt J, Bakalinsky AT. (2015) Overexpression of acetyl-CoA synthetase in Saccharomyces cerevisiae increases acetic acid tolerance. FEMS Microbiol Lett. 362(3):1-7.
  • Ding, J., Holzwarth, G., Bradford, S., Cooley, B., Yoshinaga, A.S., Patton-Vogt, J, Abeliovich, H., Penner, M.H., and Bakalinsky, A.T. (2015) PEP3 overexpression shortens lag phase but does not alter growth rate in Saccharomyces cerevisiae exposed to acetic acid stress. Appl Microbiol Biotechnol. 6/15. 1-14.
  • Surlow, B.A., Cooley, B.M., Needham, P.G., Brodsky, J.L., Patton-Vogt, J. (2014) Loss of Ypk1, the yeast homolog to the human serum- and glucocorticoid-induced protein kinase, accelerates phospholipase B1-mediated phosphatidylcholine deacylation. J. Biol. Chem. 289:31591-31604.
  • Bishop AC, Ganguly S, Solis NV, Cooley BM, Jensen-Seaman MI, Filler SG, Mitchell AP, Patton-Vogt J. Glycerophosphocholine Utilization by Candida albicans: Role of the Git3 transporter in virulence (2013) J Biol Chem. 288(47):33939-52.
  • Jun Ding, Jan Bierma, Mark Smith, Eric Poliner, Carole Wolfe, Alex Hadduck, Severino Zara, Mallori Jirikovic, Kari van Zee, Michael Penner, Jana Patton-Vogt, and Alan Bakalinsky. (2013) Acetic acid inhibits nutrient accumulation in Saccharomyces cerevisiae: auxotrophy confounds use of yeast deletion libraries for strain improvement. Appl Microbiol Biotechnol 97:7405-7416.
  • Sun T, Wetzel SJ, Johnson ME, Surlow BA, Patton-Vogt J. (2012) Development and validation of a hydrophilic interaction liquid chromatography-tandem mass spectrometry method for the quantification of lipid-related extracellular metabolites in Saccharomyces cerevisiae. J Chromatogr B Analyt Technol Biomed Life Sci. 15;897:1-9.
  • Bishop AC, Sun T, Johnson ME, Bruno VM, Patton-Vogt J (2011). Robust utilization of phospholipase-generated metabolites, glycerophosphodiesters, by Candida albicans: Role of the CaGit1 permease. Eukaryotic Cell. 10:1618-1627.
  • Ganguly S, Bishop AC, Xu W, Ghosh S, Nickerson KW, Lanni F, Patton-Vogt J, Mitchell AP (2011) Zap1 control of cell-cell signaling in Candida albicans biofilms. Eukaryotic Cell. 10:1448-1454.
  • Cheng L, Bucciarelli B, Liu J, Zinn K, Miller S, Patton-Vogt J, Allan D, Shen J, Vance CP (2011) White lupin cluster root acclimation to phosphorus deficiency and root hair development involve unique glycerophosphodiester phosphodiesterases. Plant Physiol. 156(3):1131-48.
  • Bishop, A.C., Surlow, B.A., Anand, P., Hofer, K., Henkel, M., Patton-Vogt, J. (2009) Neurofibromin homologs Ira1 and Ira2 affect glycerophosphoinositol production and transport in Saccharomyces cerevisiae, Eukaryotic Cell. 8: 1808-1811.
  • Luo J, Matsuo Y, Gulis G, Hinz H, Patton-Vogt J, Marcus S (2009) Phosphatidylethanolamine is required for normal cell morphology and cytokinesis in the fission yeast Schizosaccharomyces pombe. Eukaryot Cell. 8:790-9.
  • Nunez LR, Jesch SA, Gaspar ML, Almaguer C, Villa-Garcia M, Ruiz-Noriega M, Patton-Vogt J, Henry SA. (2008) Cell wall integrity MAPK pathway is essential for lipid homeostasis. J Biol Chem. 283:34204-17.
  • Simockova M, Holic R, Tahotna D, Patton-Vogt J, Griac P (2008) Yeast Pgc1p (YPL206c) Controls the Amount of Phosphatidylglycerol via a Phospholipase C-type Degradation Mechanism. J Biol Chem. 283:17107-17115.
  • Matsuo Y, Fisher E, Patton-Vogt J, Marcus S. (2007) Functional Characterization of the Fission Yeast Phosphatidylserine Synthase Gene, pps1, Reveals Novel Cellular Functions for Phosphatidylserine. Eukaryotic Cell 6, 2092-2101.
  • Patton-Vogt, J. (2007) Transport and metabolism of glycerophosphodiesters produced through phospholipid deacylation. Biochim. Biophys. Acta., 1771:337-342.
  • Boumann HA, J. Gubbens, M.C. Koorengevel, C.S. Oh, C.E. Martin, A.J. Heck, J. Patton-Vogt, S.A. Henry, B. de Kruijff, A.I. de Kroon (2006) Depletion of phosphatidylcholine in yeast induces shortening and increased saturation of the lipid acyl chains: evidence for regulation of intrinsic membrane curvature in a eukaryote. Mol. Biol. Cell. 17:1006-17.
  • Almaguer, C., E. Fisher, J. Patton-Vogt (2006) Posttranscriptional regulation of Git1p, the glycerophosphoinositol/glycerophosphocholine transporter of Saccharomyces cerevisiae. Curr Genet., 50(6):367-75.
  • Mariggio, S., C. Iurisci, J. Sebastia, J. Patton-Vogt and D. Corda (2006). "Molecular characterization of a glycerophosphoinositol transporter in mammalian cells." FEBS Lett 580(30): 6789-96.
  • Fisher, E., C. Almaguer, R. Holic, P. Griac, J. Patton-Vogt (2005) Glycerophosphocholine-dependent growth requires Gde1p (YPL110c) and Git1p in Saccharomyces cerevisiae. J. Biol. Chem. 280:36110-7.
  • Almaguer, C. Cheng, W. Nolder, C., and Patton-Vogt, J. Glycerophosphoinositol, a novel phosphate source whose transport is regulated by multiple factors in Saccharomyces cerevisiae. (2004) J. Biol. Chem. 279, 31937-31942.
  • Almaguer, C., Mantella, D., Perez, E., and Patton-Vogt, J. Inositol and phosphate regulate GIT1 transcription and glycerophosphoinositol incorporation in Saccharomyces cerevisiae. (2003) Eukaryotic Cell . 2, 729-736.
  • Dowd, S.R., Bier, M.E., and Patton-Vogt, J.L. Turnover of phosphatidylcholine in Saccharomyces cerevisiae (2001) J. Biol. Chem 276, 3756-3763.
  • Shirra, K.S., Patton-Vogt, J.L., Ulrich, A., Liuta-Tehlivets, O., Kohlwein, S.D., Henry, S.A., and Arndt, K.M. Inhibition of acetyl-CoA carboxylase activity restores expression of the INO1 gene in a snf1 mutant strain of Saccharomyces cerevisiae. (2001) Mol. Cell. Biol. 21, 5710-5722.
  • Srinivas, A., Patton-Vogt, J.L., Bruno, V., and Henry, S.A. A role for the major phospholipase D (Pld1p) in the secretory pathway in yeast. (1998) J. Biol. Chem 273, 16635-16638.
  • Patton-Vogt, J.L. and Henry, S.A. (1998) GIT1, a gene encoding a novel transporter for glycerophosphoinositol in Saccharomyces cerevisiae. Genetics 149, 1707-1715.
  • Henry, S.A. and Patton-Vogt, J.L. (1998) Genetic regulation of phospholipid metabolism in yeast. Progress in Nucleic Acid Research and Molecular Biology 61, 133-179. (Invited review)
  • Patton-Vogt, J.L., Griac, P., Srinivas, A., Bruno, V., Dowd, S., Swede, M. and Henry, S.A. (1997) Role of the yeast phosphatidylcholine/phosphatidylinositol transfer protein (Sec14p) in phosphatidylcholine turnover and INO1 regulation. J. Biol. Chem. 272, 20873-20883.
  • Patton, J. L., Pessoa-Brandao, L., and Henry, S. A. (1995) Production and utilization of an extracellular phosphatidylinositol catabolite, glycerophosphoinositol, by Saccharomyces cerevisiae. J. Bacteriol. 177, 3379-3385.
  • BIOL212          Cell and Molecular Biology
  • BIOL371W       Lab II Cell and Molecular Biology
  • BIOL647          Advanced Cell & Molecular Biology II
I am the faculty sponsor for the Duquesne Student Chapter of the  American Society of Biochemistry and Molecular Biology  (ASBMB). The ASBMB student chapters comprise a national organization of university-based chapters dedicated to the advancement of undergraduate research and education in biochemistry and molecular biology. Student members receive membership to ASBMB, access to ASBMB-related journals, and free access to other publications on topics such as graduate education and career choices. In addition, student members are eligible for awards and scholarships for research and travel.