Melikhan Tanyeri, Ph.D.

My current research endeavors revolve around the application of micro and nanoscale tools/systems, coupled with cutting-edge optical microscopy techniques, to address complex challenges in biosensors, point-of-care diagnostics, and the mechanics & dynamics of biomolecules and cells. Within my research group, we possess extensive expertise in microfabrication, micropatterning, soft lithography microfluidics, as well as proficiency in brightfield, fluorescence, and superresolution microscopy. Our focus spans cell-material interactions, development & applications of novel sensors and actuators, and the design and implementation of methods, assays, and devices at the micro- and nanoscale.

Some ongoing projects within our research portfolio include:

• Development of portable blood coagulation analysis systems.
• Design and utilization of microfluidic platforms for studying single-cell mechanotransduction.
• Exploration of microfluidic trapping, manipulation, and separation techniques for biomolecules and cells.
• High throughput analysis of single cell behavior.
• Early detection methods for bacteria.

In essence, our multidisciplinary and applied research program centers on the creation of optofluidic tools and techniques to tackle current challenges in the biomedical field. My specific research interests encompass:

• Biomedical microdevices, micro and nanosystems for biology and medicine.
• Microfluidics, bioMEMS/NEMS, in vitro diagnostics, lab-on-a-chip, medical devices, and biosensors.
• Detection and manipulation techniques for biomolecules, cells, and microorganisms.
• Microfabrication, soft lithography, and micropatterning.
• Superresolution imaging and fluorescence spectroscopy.

I adhere to the "educate, inspire, engage" philosophy in my approach to teaching and advising. My primary goal in these roles is to cultivate a positive learning environment that encourages students to acquire, test, and generate knowledge, equipping them with essential skills to emerge as distinguished individuals among the next generation of engineers. As a teacher and mentor, I envision my role as one that kindles students' enthusiasm, stimulates their interest, and ultimately empowers them to think creatively and independently.

Within the domain of Biomedical Engineering, I instruct the following courses under both our undergraduate and graduate programs:

• BMED 110 Introduction to Programming for Engineers
• BMED 310 Biomedical Signals and Systems
• BMED 451/551 Biomedical Microdevices I
• BMED 452/552 Biomedical Microdevices II

Synopsis: 2 book chapters, 20 journal articles, 11 conference proceedings, 4 patents
Web of Science: h-index: 12, total citations: 683, total publications: 32
Scopus: h-index: 13, total citations: 727, total publications: 27
Google Scholar: h-index: 14, total citations: 1054, total publications: 53

Book Chapter:

1. Tanyeri M, and Tay S
“Viable cell culture in PDMS-based microfluidic devices”
Methods in Cell Biology, Volume 148
Microfluidics in Cell Biology Part C: Microfluidics for Cellular and Subcellular Analysis (2018)
Editors: Daniel A. Fletcher, Junsang Doh and Matthieu Piel, ISBN: 978-0-12-814284-4, Elsevier.

2. Tanyeri M, and Schroeder CM
“Flow-based particle trapping and manipulation”
Encyclopedia of Microfluidics and Nanofluidics (2014) Article ID: 347830, Chapter ID: 1770 (ed. Dongqing Li), Springer.

Journal:

1. Mustafa A, Ertas Uslu M, and Tanyeri M
“Optimizing Sensitivity in a Fluid-Structure Interaction-Based Microfluidic Viscometer: A Multiphysics Simulation Study”
Sensors (2023) 23(22), 9265. DOI: https://doi.org/10.3390/s23229265

2. Yang B, Schinke J, Rastegar A, Tanyeri M, and Viator JA
“Cost-Effective Full-Color 3D Dental Imaging Based on Close-Range Photogrammetry”
Bioengineering (2023) 10(11), 1268. DOI: 10.3390/bioengineering10111268

3. Mustafa A, Haider D, Barua A, Tanyeri M, Erten A, and Yalcin O
“Machine learning based microfluidic sensing device for viscosity measurements”
Sensors and Diagnostics (2023) 2, 1509-1520. DOI: 10.1039/d3sd00099k

4. Boyd J, Hepner G, Ujhazy M, Bliss S, and Tanyeri M
“Dual hydrodynamic trap based on coupled stagnation point flows”
Physics of Fluids (2023) 35, 062001. DOI: 10.1063/5.0150089

5. Mustafa A, Eser A, Aksu AC, Kiraz A, Tanyeri M, Erten A, and Yalcin O
“A micropillar-based microfluidic viscometer for Newtonian and non-Newtonian fluids”
Analytica Chimica Acta (2020) 1135 107-115.

6. Watterson WJ, Tanyeri M, Watson AR, Cham CM, Shan Y, Chang EB, Eren AM, and Tay S
“Droplet-based high-throughput cultivation for accurate screening of antibiotic resistant gut microbes”
eLife (2020) 9:e56998.

7. Evans A, Sutton K, Hernandez S, and Tanyeri M
“Viscoelastic Hemostatic Assays - A Quest for Holy Grail of Coagulation Monitoring in Trauma Care”
Journal of Annals of Bioengineering (2019) 1: 61-64.

8. Mustafa A, Erten A, Ayaz R, Kayillioglu O, Eser A, Irfan M, Muradoglu M, Tanyeri M, and Kiraz A
“Enhanced dissolution of liquid microdroplets in the extensional creeping flow of a hydrodynamic trap”
Langmuir (2016) 32 (37) 9460-9467.

9. Shenoy A, Tanyeri M, and Schroeder CM
“Characterizing the performance of the hydrodynamic trap using a control-based approach”
Microfluidics and Nanofluidics (2015) 18 (5) 1055-1066.

10. Johnson-Chavarria EM, Agrawal U, Tanyeri M, Kuhlman TE, and Schroeder CM
“Automated single cell microbioreactor for monitoring intracellular dynamics and cell growth in free solution”
Lab on a Chip (2014) 14 (15) 2688-2697.

11. Marciel AB, Tanyeri M, Wall BD, Tovar JD, Schroeder CM, and Wilson WL
“Fluidic-directed assembly of aligned oligopeptides with π-conjugated cores”
Advanced Materials (2013) 25 (44) 6398-6404.

12. Tanyeri M, and Schroeder CM
“Manipulation and confinement of single particles using fluid flow”
Nano Letters (2013) 13 (6) 2357-2364.

13. Kim Y, Kim SH, Tanyeri M, Katzenellenbogen JA, and Schroeder CM
“Dendrimer probes for enhanced photostability and localization in fluorescence imaging”
Biophysical Journal (2013) 104 (7) 1566-1575.

14. Tanyeri M, Ranka M, Sittipolkul N, and Schroeder CM
“Microfluidic Wheatstone bridge for rapid sample analysis”
Lab on a Chip (2011) 11 (24) 4181-4186.

15. Tanyeri M, Ranka M, Sittipolkul N, and Schroeder CM
“A microfluidic-based hydrodynamic trap: Design and implementation”
Lab on a Chip (2011) 11 (10) 1786-1794.
(selected for the May 23, 2011 issue of Virtual Journal of Nanoscale Science & Technology)

16. Schudel BR, Tanyeri M, Mukherjee A, Schroeder CM, and Kenis PJA
“Multiplexed detection of nucleic acids in a combinatorial screening chip”
Lab on a Chip (2011) 11 (11) 1916-1923.

17. Johnson-Chavarria EM, Tanyeri M, and Schroeder CM
“A microfluidic-based hydrodynamic trap for single particles”
Journal of Visualized Experiments (2011) (47) DOI: 10.3791/2517.

18. Tanyeri M, Johnson-Chavarria EM, and Schroeder CM
“Hydrodynamic trap for single particles and cells”
Applied Physics Letters (2010) 96 224101.
(selected for the June 14, 2010 issue of Virtual Journal of Nanoscale Science & Technology)

19. Tanyeri M, and Kennedy IM
“Detecting single bacterial cells through optical resonances in microdroplets”
Sensor Letters (2008) Vol. 6 No. 2 p. 326-329.
(cover article)

20. Tanyeri M, Perron R, and Kennedy IM
“Lasing droplets in a microfabricated channel”
Optics Letters (2007) Vol. 32 Issue 17 p. 2529-2531.
(highlighted in photonics.com and Photonics Spectra, October 2007)

Conference Proceedings:

1. Hoying M, Kazimer B, Evans A, Carbino B, Sutton K, McCallin R, and McGee A
“Project MADMEN: Proposed Analogue Fidelity Comparison to ALIEN Martian Mission”
2022 IEEE Aerospace Conference (AERO) (2022) pp. 01-10, DOI: 10.1109/AERO53065.2022.9843386.

2. Kayillioglu O, Erten A, Tanyeri M, and Kiraz A
“Dye Lasing and Laminar Flow-Induced Dissolution in Hydrodynamically Trapped Oil Microdroplets”
OSA Technical Digest (2015) pp. OtW2D.4 Optical Trapping Applications 2015.

3. Kayillioglu O, Erten A, Kiraz A and Tanyeri M
“Hydrodynamic Trapping of Oil Microdroplets in Glycerol-Water Solution”
Proc. BiyoMUT (2014) BIYOMUT 2014: 18th National Biomedical Engineering Meeting, Istanbul, Turkey.

4. Tanyeri M, and Schroeder CM
“Confinement and manipulation of single nanoparticles in free solution using a hydrodynamic trap”
Proc. NanoTR (2014) 10th Nanoscience and Nanotechnology Conference, Istanbul, Turkey.

5. Tanyeri M
“Confinement of single macromolecules in free solution using a hydrodynamic trap”
Proc. SPIE (2014) Vol. 8976 p. 89760F Microfluidics, BioMEMS, and Medical Microsystems XII.

6. Tanyeri M, and Schroeder CM
“2-D micromanipulation of single nanoparticles in free solution using a microfluidic trap”
Proc. MicroTAS (2011) 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences.

7. Schudel BR, Tanyeri M, Schroeder CM, and Kenis PJA
“Total internal reflection fluorescence of molecular beacons in a multiplexed microfluidic device”
Proc. MicroTAS (2009) Vol. 1 p. 36-38 13th International Conference on Miniaturized Systems for Chemistry and Life
Sciences.

8. Tanyeri M, Perron R, and Kennedy IM
“Lasing droplets in a microfluidic T-junction device with integrated optics”
Proc. SPIE (2007) Vol. 6465 p. 64650E Microfluidics, BioMEMS, and Medical Microsystems V.

9. Tanyeri M, Dosev DK, and Kennedy IM
“Chemical and biological sensing through optical resonances in pendant droplets”
Proc. SPIE (2005) Vol. 6008 p. 60080Q Nanosensing: Materials and Devices II.

10. Tanyeri M, Nichkova M, Hammock BD, and Kennedy IM
“Chemical and biological sensing through optical resonances in microcavities”
Proc. SPIE (2005) Vol. 5699 p. 227-236 Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals
and Applications III.

11. Tanyeri M, and Kennedy IM
“Microdroplets for integrated high-sensitivity biosensors”
Proc. SPIE (2004) Vol. 5275 p. 133-140 BioMEMS and Nanotechnology.

Patents:

1. Tanyeri M, Lin J, Abasiyanik F, Angarita Marmolejo YD
“Rapid Enumeration of Microorganisms”
US Application No: 63/184,447 (May 5, 2021), WO 2022/235284 (Nov 10, 2022).

2. Yalcin O, Erten AC, Tanyeri M
“Microfluidic Thromboelastometry Instrument”
US2021/0268497 (Sep 2, 2021), WO 2020/027741 (Feb 6, 2020), EP3830573 (Jul 29, 2018).

3. Kim Y, Kim SH, Tanyeri M, Katzenellenbogen JA, and Schroeder CM
“Dye-conjugated dendrimers”
US Patent 9,448,173 (September 20 2016).

4. Tanyeri M, Perron R, and Kennedy IM
“Optical resonances in droplets in a microchannel”
WIPO Publication No: WO 2008/030281 (March 13 2008). International Application No: PCT/US2007/012590.

Presentations
Selected recent presentations are listed below:

Invited:

1. “Microfluidic techniques for probing molecular and cellular processes”, Duquesne University, Biological Sciences Seminar, Pittsburgh, PA (October 27, 2023)

2. “Microfluidic tools for studying biomolecules, cells and soft matter”, Ohio State University, Biophysics Seminar, Columbus, OH (September 29, 2021)

3. “Hydrodynamic Trap: A new microfluidic tool for studying soft matter”, Carnegie Mellon University, Colloids, Polymers and Surfaces Seminar, Pittsburgh, PA (March 9, 2018)

4. “Observation of droplet dissolution in aqueous media using a hydrodynamic trap”, 3rd World Chemistry Conference, Dallas, TX (September 11-12, 2017)


Contributed:


1. Biomedical Engineering Society (BMES) Annual Meeting (poster presentation), “A Microfluidic Tweezer for Simultaneous Confinement of a Pair of Cells and Particles”, Jarrett Boyd, Gram Hepner, Maxwell Ujhazy, Shawn Bliss, Melikhan Tanyeri, Seattle, WA (Oct 11-14, 2023)

2. ConnectUR 2023 Annual Conference (oral presentation), “A senior-level course in biomedical engineering to develop skills for comprehensive analysis of primary scientific literature and communication of science to the public”, Melikhan Tanyeri, Duquesne University (June 26-28, 2023)

3. Biomedical Engineering Society (BMES) Annual Meeting (poster presentation) “Application of Supervised Machine Learning for Bacteria Analysis in Droplet Microfluidics”, Shawn Bliss, Melikhan Tanyeri, San Antonio, TX (Oct 12-15, 2022)

4. Biomedical Engineering Society (BMES) Annual Meeting (poster presentation) “PsychLight: a Genetically Encoded Fluorescent Sensor for Drug Discovery” Caroline Daggett, Asef Faruk, Jane Cavanaugh, Kevin Tidgewell, Melikhan Tanyeri, San Antonio, TX (Oct 12-15, 2022)

5. Biomedical Engineering Society (BMES) Annual Meeting (poster presentation) “A Microfluidic Viscometer based on Micropillar Deflection and Machine Learning”, Adil Mustafa, Daniyal Haider, Arnab Barua, Melikhan Tanyeri, Ahmet Erten, Ozlem Yalcin, San Antonio, TX (Oct 12-15, 2022)

6. National Conference on Undergraduate Research (NCUR) 2022, “Microfluidic-Photoacoustic Flow Cytometry for Diagnosis of Acute Lymphocytic Leukemia”, Shawn Bliss, Melikhan Tanyeri, Virtual, (April 4-8, 2022)

7. Biomedical Engineering Society (BMES) Annual Meeting (oral), “A Microfluidic Viscometer towards Real-time, Continuous Measurement of Blood Viscosity”, Melikhan Tanyeri, Adil Mustafa, Ahmet Erten, Ozlem Yalcin, Orlando, FL (Oct 6-9, 2021)

8. American Institute of Chemical Engineers Annual Meeting (oral), “Dual Hydrodynamic Trap”, San Francisco, CA (November 15-20, 2020)

9. Virtual EMBL Conference: Microfluidics: Designing the Next Wave of Biological Inquiry (poster) “Dual particle trapping using a microfluidic trap” Presenter: Jarrett Boyd, Virtual/Heidelberg, Germany (July 13-15, 2020)